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tieclipse inverted widefield epifluorescent microscope  (Nikon)


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    Structured Review

    Nikon tieclipse inverted widefield epifluorescent microscope
    Tieclipse Inverted Widefield Epifluorescent Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tieclipse+inverted+epifluorescent+microscope/10__1091_slash_mbc__e18___09___0590-233-5-4
    Average 90 stars, based on 1 article reviews
    tieclipse inverted widefield epifluorescent microscope - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: A patient-derived cellular model for Huntington’s disease reveals phenotypes at clinically relevant CAG lengths
    Article Snippet: Immunofluorescence Peptide Competition Assay for Antibody Validation The N17-phospho antibody (1:250) was incubated, with rotation, with 1000 ng of synthetic N17 peptides (N17, N17S13p, N17S16p, N17S13pS16p, and a control peptide – p53 (371-393); New England Peptides) at room temperature for 1 hour prior to overnight incubation with RPE1 cells fixed with methanol. .. Cells were washed 3 times with 2% FBS in PBS and then incubated in anti-rabbit AlexaFluor488 secondary antibody (1:500, Molecular Probes) for 45 minutes at room temperature and then washed and left in PBS before imaging using a Nikon TiEclipse inverted epifluorescent microscope. .. Endogenous huntingtin knockdown was established with huntingtin siRNA (Santa Cruz, sc35617) in RPE1 cells. siRNA was transfected using Lipofectamine RNAiMax (Invitrogen) according to manufacturer instructions.

    Article Title: A Patient-Derived Cellular Model for Huntington’s Disease Reveals Phenotypes at Clinically Relevant CAG Lengths
    Article Snippet: The N17-phospho antibody (1:250) was incubated, with rotation, with 1000 ng of synthetic N17 peptides (N17, N17S13p, N17S16p, N17S13pS16p, and a control peptide – p53 (371-393); New England Peptides) at room temperature for 1 hour prior to overnight incubation with RPE1 cells fixed with methanol. .. Cells were washed 3 times with 2% FBS in PBS and then incubated in anti-rabbit AlexaFluor488 secondary antibody (1:500, Molecular Probes) for 45 minutes at room temperature and then washed and left in PBS before imaging using a Nikon TiEclipse inverted epifluorescent microscope. .. Endogenous huntingtin knockdown was established with huntingtin siRNA (Santa Cruz, sc35617) in RPE1 cells. siRNA was transfected using Lipofectamine RNAiMax (Invitrogen) according to manufacturer instructions.

    Imaging:

    Article Title: A patient-derived cellular model for Huntington’s disease reveals phenotypes at clinically relevant CAG lengths
    Article Snippet: Immunofluorescence Peptide Competition Assay for Antibody Validation The N17-phospho antibody (1:250) was incubated, with rotation, with 1000 ng of synthetic N17 peptides (N17, N17S13p, N17S16p, N17S13pS16p, and a control peptide – p53 (371-393); New England Peptides) at room temperature for 1 hour prior to overnight incubation with RPE1 cells fixed with methanol. .. Cells were washed 3 times with 2% FBS in PBS and then incubated in anti-rabbit AlexaFluor488 secondary antibody (1:500, Molecular Probes) for 45 minutes at room temperature and then washed and left in PBS before imaging using a Nikon TiEclipse inverted epifluorescent microscope. .. Endogenous huntingtin knockdown was established with huntingtin siRNA (Santa Cruz, sc35617) in RPE1 cells. siRNA was transfected using Lipofectamine RNAiMax (Invitrogen) according to manufacturer instructions.

    Article Title: A Patient-Derived Cellular Model for Huntington’s Disease Reveals Phenotypes at Clinically Relevant CAG Lengths
    Article Snippet: The N17-phospho antibody (1:250) was incubated, with rotation, with 1000 ng of synthetic N17 peptides (N17, N17S13p, N17S16p, N17S13pS16p, and a control peptide – p53 (371-393); New England Peptides) at room temperature for 1 hour prior to overnight incubation with RPE1 cells fixed with methanol. .. Cells were washed 3 times with 2% FBS in PBS and then incubated in anti-rabbit AlexaFluor488 secondary antibody (1:500, Molecular Probes) for 45 minutes at room temperature and then washed and left in PBS before imaging using a Nikon TiEclipse inverted epifluorescent microscope. .. Endogenous huntingtin knockdown was established with huntingtin siRNA (Santa Cruz, sc35617) in RPE1 cells. siRNA was transfected using Lipofectamine RNAiMax (Invitrogen) according to manufacturer instructions.

    Microscopy:

    Article Title: A patient-derived cellular model for Huntington’s disease reveals phenotypes at clinically relevant CAG lengths
    Article Snippet: Immunofluorescence Peptide Competition Assay for Antibody Validation The N17-phospho antibody (1:250) was incubated, with rotation, with 1000 ng of synthetic N17 peptides (N17, N17S13p, N17S16p, N17S13pS16p, and a control peptide – p53 (371-393); New England Peptides) at room temperature for 1 hour prior to overnight incubation with RPE1 cells fixed with methanol. .. Cells were washed 3 times with 2% FBS in PBS and then incubated in anti-rabbit AlexaFluor488 secondary antibody (1:500, Molecular Probes) for 45 minutes at room temperature and then washed and left in PBS before imaging using a Nikon TiEclipse inverted epifluorescent microscope. .. Endogenous huntingtin knockdown was established with huntingtin siRNA (Santa Cruz, sc35617) in RPE1 cells. siRNA was transfected using Lipofectamine RNAiMax (Invitrogen) according to manufacturer instructions.

    Article Title: A Patient-Derived Cellular Model for Huntington’s Disease Reveals Phenotypes at Clinically Relevant CAG Lengths
    Article Snippet: The N17-phospho antibody (1:250) was incubated, with rotation, with 1000 ng of synthetic N17 peptides (N17, N17S13p, N17S16p, N17S13pS16p, and a control peptide – p53 (371-393); New England Peptides) at room temperature for 1 hour prior to overnight incubation with RPE1 cells fixed with methanol. .. Cells were washed 3 times with 2% FBS in PBS and then incubated in anti-rabbit AlexaFluor488 secondary antibody (1:500, Molecular Probes) for 45 minutes at room temperature and then washed and left in PBS before imaging using a Nikon TiEclipse inverted epifluorescent microscope. .. Endogenous huntingtin knockdown was established with huntingtin siRNA (Santa Cruz, sc35617) in RPE1 cells. siRNA was transfected using Lipofectamine RNAiMax (Invitrogen) according to manufacturer instructions.



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